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b 3 spectrin  (Novus Biologicals)


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    Novus Biologicals b 3 spectrin
    Figure 7. <t>AnkR-spectrin</t> interactions in the cerebellum. A, Immunostaining of sagittal cerebellum for AnkR (polyclonal, red) and b 1 spectrin (monoclonal, green). Scale bars: 20mm. B, Immunostaining of sagittal cerebellum for AnkR (monoclonal, red) and b 3 spectrin (polyclonal, green). Scale bars: 20mm. C, Immunostaining of sagittal cerebellum for b 1 spectrin in three- month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50 mm. D, Immunostaining of sagittal cerebellum for b 3 spectrin in three-month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50mm. E, Quantification of b 1 spectrin fluorescence intensity in the granule cell layer (GCL) of three-month Ank1F/F (N = 3; 686 6.1 AU) and Ank1F/F;Nestin-Cre (N = 3; 61 6 3.8 AU). F, Quantification of b 1 spectrin fluorescence intensity at the Purkinje neuron soma by measuring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 396 2.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 38 6 1.6 AU). Error bars indicate mean 6 SEM. G, Quantification of b 3 spectrin fluorescence intensity in the molecular layer (ML) of three-month Ank1F/F (N = 3; 63 6 2.2 AU) and Ank1F/F;Nestin-Cre (N = 3; 666 3.1 AU). H, Quantification of b 3 spectrin fluorescence intensity at the Purkinje neuron soma by meas- uring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 52 6 4.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 406 3.9 AU). Error bars indicate mean 6 SEM. I, Immunoblots of b 3 spectrin, AnkR, and IgG immunoprecipitation (IP) reactions using antibodies against AnkR and b 3 spectrin.
    B 3 Spectrin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b 3 spectrin/product/Novus Biologicals
    Average 94 stars, based on 5 article reviews
    b 3 spectrin - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival"

    Article Title: Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival

    Journal: The Journal of Neuroscience

    doi: 10.1523/jneurosci.1132-21.2021

    Figure 7. AnkR-spectrin interactions in the cerebellum. A, Immunostaining of sagittal cerebellum for AnkR (polyclonal, red) and b 1 spectrin (monoclonal, green). Scale bars: 20mm. B, Immunostaining of sagittal cerebellum for AnkR (monoclonal, red) and b 3 spectrin (polyclonal, green). Scale bars: 20mm. C, Immunostaining of sagittal cerebellum for b 1 spectrin in three- month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50 mm. D, Immunostaining of sagittal cerebellum for b 3 spectrin in three-month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50mm. E, Quantification of b 1 spectrin fluorescence intensity in the granule cell layer (GCL) of three-month Ank1F/F (N = 3; 686 6.1 AU) and Ank1F/F;Nestin-Cre (N = 3; 61 6 3.8 AU). F, Quantification of b 1 spectrin fluorescence intensity at the Purkinje neuron soma by measuring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 396 2.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 38 6 1.6 AU). Error bars indicate mean 6 SEM. G, Quantification of b 3 spectrin fluorescence intensity in the molecular layer (ML) of three-month Ank1F/F (N = 3; 63 6 2.2 AU) and Ank1F/F;Nestin-Cre (N = 3; 666 3.1 AU). H, Quantification of b 3 spectrin fluorescence intensity at the Purkinje neuron soma by meas- uring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 52 6 4.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 406 3.9 AU). Error bars indicate mean 6 SEM. I, Immunoblots of b 3 spectrin, AnkR, and IgG immunoprecipitation (IP) reactions using antibodies against AnkR and b 3 spectrin.
    Figure Legend Snippet: Figure 7. AnkR-spectrin interactions in the cerebellum. A, Immunostaining of sagittal cerebellum for AnkR (polyclonal, red) and b 1 spectrin (monoclonal, green). Scale bars: 20mm. B, Immunostaining of sagittal cerebellum for AnkR (monoclonal, red) and b 3 spectrin (polyclonal, green). Scale bars: 20mm. C, Immunostaining of sagittal cerebellum for b 1 spectrin in three- month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50 mm. D, Immunostaining of sagittal cerebellum for b 3 spectrin in three-month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50mm. E, Quantification of b 1 spectrin fluorescence intensity in the granule cell layer (GCL) of three-month Ank1F/F (N = 3; 686 6.1 AU) and Ank1F/F;Nestin-Cre (N = 3; 61 6 3.8 AU). F, Quantification of b 1 spectrin fluorescence intensity at the Purkinje neuron soma by measuring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 396 2.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 38 6 1.6 AU). Error bars indicate mean 6 SEM. G, Quantification of b 3 spectrin fluorescence intensity in the molecular layer (ML) of three-month Ank1F/F (N = 3; 63 6 2.2 AU) and Ank1F/F;Nestin-Cre (N = 3; 666 3.1 AU). H, Quantification of b 3 spectrin fluorescence intensity at the Purkinje neuron soma by meas- uring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 52 6 4.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 406 3.9 AU). Error bars indicate mean 6 SEM. I, Immunoblots of b 3 spectrin, AnkR, and IgG immunoprecipitation (IP) reactions using antibodies against AnkR and b 3 spectrin.

    Techniques Used: Immunostaining, Fluorescence, Western Blot, Immunoprecipitation



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    b 3 spectrin  (Novus Biologicals)
    94
    Novus Biologicals b 3 spectrin
    Figure 7. <t>AnkR-spectrin</t> interactions in the cerebellum. A, Immunostaining of sagittal cerebellum for AnkR (polyclonal, red) and b 1 spectrin (monoclonal, green). Scale bars: 20mm. B, Immunostaining of sagittal cerebellum for AnkR (monoclonal, red) and b 3 spectrin (polyclonal, green). Scale bars: 20mm. C, Immunostaining of sagittal cerebellum for b 1 spectrin in three- month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50 mm. D, Immunostaining of sagittal cerebellum for b 3 spectrin in three-month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50mm. E, Quantification of b 1 spectrin fluorescence intensity in the granule cell layer (GCL) of three-month Ank1F/F (N = 3; 686 6.1 AU) and Ank1F/F;Nestin-Cre (N = 3; 61 6 3.8 AU). F, Quantification of b 1 spectrin fluorescence intensity at the Purkinje neuron soma by measuring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 396 2.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 38 6 1.6 AU). Error bars indicate mean 6 SEM. G, Quantification of b 3 spectrin fluorescence intensity in the molecular layer (ML) of three-month Ank1F/F (N = 3; 63 6 2.2 AU) and Ank1F/F;Nestin-Cre (N = 3; 666 3.1 AU). H, Quantification of b 3 spectrin fluorescence intensity at the Purkinje neuron soma by meas- uring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 52 6 4.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 406 3.9 AU). Error bars indicate mean 6 SEM. I, Immunoblots of b 3 spectrin, AnkR, and IgG immunoprecipitation (IP) reactions using antibodies against AnkR and b 3 spectrin.
    B 3 Spectrin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b 3 spectrin/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    b 3 spectrin - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 7. AnkR-spectrin interactions in the cerebellum. A, Immunostaining of sagittal cerebellum for AnkR (polyclonal, red) and b 1 spectrin (monoclonal, green). Scale bars: 20mm. B, Immunostaining of sagittal cerebellum for AnkR (monoclonal, red) and b 3 spectrin (polyclonal, green). Scale bars: 20mm. C, Immunostaining of sagittal cerebellum for b 1 spectrin in three- month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50 mm. D, Immunostaining of sagittal cerebellum for b 3 spectrin in three-month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50mm. E, Quantification of b 1 spectrin fluorescence intensity in the granule cell layer (GCL) of three-month Ank1F/F (N = 3; 686 6.1 AU) and Ank1F/F;Nestin-Cre (N = 3; 61 6 3.8 AU). F, Quantification of b 1 spectrin fluorescence intensity at the Purkinje neuron soma by measuring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 396 2.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 38 6 1.6 AU). Error bars indicate mean 6 SEM. G, Quantification of b 3 spectrin fluorescence intensity in the molecular layer (ML) of three-month Ank1F/F (N = 3; 63 6 2.2 AU) and Ank1F/F;Nestin-Cre (N = 3; 666 3.1 AU). H, Quantification of b 3 spectrin fluorescence intensity at the Purkinje neuron soma by meas- uring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 52 6 4.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 406 3.9 AU). Error bars indicate mean 6 SEM. I, Immunoblots of b 3 spectrin, AnkR, and IgG immunoprecipitation (IP) reactions using antibodies against AnkR and b 3 spectrin.

    Journal: The Journal of Neuroscience

    Article Title: Ankyrin-R Links Kv3.3 to the Spectrin Cytoskeleton and Is Required for Purkinje Neuron Survival

    doi: 10.1523/jneurosci.1132-21.2021

    Figure Lengend Snippet: Figure 7. AnkR-spectrin interactions in the cerebellum. A, Immunostaining of sagittal cerebellum for AnkR (polyclonal, red) and b 1 spectrin (monoclonal, green). Scale bars: 20mm. B, Immunostaining of sagittal cerebellum for AnkR (monoclonal, red) and b 3 spectrin (polyclonal, green). Scale bars: 20mm. C, Immunostaining of sagittal cerebellum for b 1 spectrin in three- month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50 mm. D, Immunostaining of sagittal cerebellum for b 3 spectrin in three-month Ank1F/F and Ank1F/F;Nestin-Cre. Scale bar: 50mm. E, Quantification of b 1 spectrin fluorescence intensity in the granule cell layer (GCL) of three-month Ank1F/F (N = 3; 686 6.1 AU) and Ank1F/F;Nestin-Cre (N = 3; 61 6 3.8 AU). F, Quantification of b 1 spectrin fluorescence intensity at the Purkinje neuron soma by measuring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 396 2.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 38 6 1.6 AU). Error bars indicate mean 6 SEM. G, Quantification of b 3 spectrin fluorescence intensity in the molecular layer (ML) of three-month Ank1F/F (N = 3; 63 6 2.2 AU) and Ank1F/F;Nestin-Cre (N = 3; 666 3.1 AU). H, Quantification of b 3 spectrin fluorescence intensity at the Purkinje neuron soma by meas- uring the fluorescence intensity of a line across the PCL and dividing by length in three-month sagittal cerebellum of Ank1F/F (N = 3; 52 6 4.4 AU) and Ank1F/F;Nestin-Cre (N = 3; 406 3.9 AU). Error bars indicate mean 6 SEM. I, Immunoblots of b 3 spectrin, AnkR, and IgG immunoprecipitation (IP) reactions using antibodies against AnkR and b 3 spectrin.

    Article Snippet: The primary antibodies used here include: mouse monoclonal antibodies against AnkR (IF: 1:250, UC Davis/NIH NeuroMab Facility catalog #75- 380, RRID:AB_2491109), b 1 spectrin (IF: 1:250, UC Davis/NIH NeuroMab Facility catalog #73-374, RRID:AB_2315814), AnkG (IF: 1:500, UC Davis/NIH NeuroMab Facility catalog #73-146, RRID:AB_ 10697718), Kv3.3 (IF: 1:500, Antibodies-Online catalog #ABIN572016, RRID:AB_10782137), Calbindin (IF: 1:250, UC Davis/NIH NeuroMab Facility catalog #L109/57, RRID:AB_2877197), and Flag-tag or DDDDK-tag (WB: 1:1000, MBL International catalog #M185-3L, RRID: AB_11123930), rabbit polyclonal antibodies against AnkR (IF: 1:500, WB: 1:1000, RRID:AB_2833096), Ank1 (IF: 1:500, WB: 1:1000, Thermo Fisher Scientific catalog #PA5-63372, RRID:AB_2638015), AnkB (IF: 1:250, Santa Cruz Biotechnology catalog #sc-28560, RRID:AB_2242828), b 3 spectrin (IF: 1:500, WB: 1:1000, Novus catalog #NB110-58346, RRID:AB_877723), Calbindin D-28k (IF: 1:500, Swant catalog #CB38, RRID:AB_10000340), Kv3.3 (IF: 1:500, WB: 1:1000, Alomone Labs catalog #APC-102, RRID:AB_2040170), and GFP (WB: 1:1000, Thermo Fisher Scientific, catalog #A-11122, RRID:AB_221569).

    Techniques: Immunostaining, Fluorescence, Western Blot, Immunoprecipitation